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The initiation and subsequent development of laticifers werestudied in callus tissues of Calotropis gigantea grown on MS(Murashige and Skoog) medium supplemented with 1 mg l1IAA. Laticifer development was related to the age of the cultureand could be preserved by repeated subculturing on similar mediumwith IAA. Our previous work had established that cardenolidebiosynthesis was related to rhizogenesis and here we reportthe culture system of laticifer, long term preservation anddifferentiation of this hydrocarbon producing energy plant. Calotropis gigantea, Asclepiadaceae, laticifer, differentiation, cardenolides, tissue culture 相似文献